Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Fibroblast growth factor-inducible 14 accelerates pulmonary fibrosis by inducing fibroblast senescence in mice

doi: 10.1007/s00018-026-06161-w

Figure Lengend Snippet: Fn14 shRNA alleviates BLM-induced PF in mice. A , C57BL/6J mice received empty adenovirus or Fn14 vectors (5 × 10 8 PFU/20 g, i.t. ). Seven days later, mice were administered BLM (2.5 mg/kg, i.t. ) to establish a PF model. Mice were sacrificed after the BLM administration on the 21st day. B , the expression of Fn14 mRNA in the lungs was detected by Real-time PCR. C-D , the expression of Fn14 protein in the lungs was detected by Western blot ( n = 6–8). E, weight change of mice ( n = 6–10). F, the rate of weight change in mice was calculated. G, the pulmonary tissue histopathology of mice was stained with H&E (Top row), and the collagen deposition was detected by Masson’s trichrome staining (Bottom row). H, the Ashcroft score was evaluated by three blinded pathologists. I, the collagen subtype was detected by Sirius red staining. J, the expressions of Col1a1 , Col3a1 , and Acta2 mRNA in the lungs were detected by Real-time PCR. L, the expressions of Collagen I, Collagen III, and α-SMA proteins in the lungs were assayed by Western blot ( n = 6–8). ***P < 0.001

Article Snippet: Anti-α-SMA polyclonal antibody , CST , 19,245 , 1: 5000.

Techniques: shRNA, Expressing, Real-time Polymerase Chain Reaction, Western Blot, Histopathology, Staining